Expression of RPRM/rprm in the Olfactory System of Embryonic Zebrafish (Danio rerio)

ABSTRACT

The Reprimo (RPRM) family is composed of highly conserved single-exon genes The expression pattern of this gene family has been recently described during zebrafish (Danio rerio) embryogenesis, and primarily locates in the nervous system Its most characterized member, RPRM, which duplicated to give rise rprma and rprmb in the fish lineage, is known to act as a tumor-suppressor gene in mammalian models Here, we describe in detail the spatiotemporal expression of three rprm genes (rprma, rprmb, and rprml) within distinct anatomical structures in the developing peripheral and central nervous system In the zebrafish, rprma mRNA is expressed in the olfactory placodes (OP) and olfactory epithelium (OE), rprmb is observed in the tectum opticum (TeO) and trigeminal ganglion (Tg), whereas rprml is found primarily in the telencephalon (Tel) At protein level, RPRM is present in a subset of cells in the OP, and neurons in the OE, TeO, hindbrain and sensory peripheral structures Most importantly, the expression of RPRM has been conserved between teleosts and mammals Thus, we provide a reference dataset describing the expression patterns of RPRM gene products during zebrafish and mouse development as a first step to approach the physiological role of the RPRM gene family

Stanic K, Quiroz A, Lemus CG, Wichmann IA, Corvalán AH, Owen GI, Opazo JC, Concha ML, Amigo JD

Frontiers In Neuroanatomy (Front Neuroanat)

marzo 27, 2018

DOI: 103389/fnana201800023

Investigador BNI: Miguel Concha