Dopamine receptor D3 signalling in astrocytes promotes neuroinflammation.

ABSTRACT

Background Neuroinflammation constitutes a pathogenic process leading to neurodegeneration in several disorders, including Alzheimer’s disease, Parkinson’s disease (PD) and sepsis. Despite microglial cells being the central players in neuroinflammation, astrocytes play a key regulatory role in this process. Our previous results indicated that pharmacologic-antagonism or genetic deficiency of dopamine receptor D3 (DRD3) attenuated neuroinflammation and neurodegeneration in two mouse models of PD. Here, we studied how DRD3-signalling affects the dynamic of activation of microglia and astrocyte in the context of systemic inflammation. Methods Neuroinflammation was induced by intraperitoneal administration of LPS. The effect of genetic DRD3-deficiency or pharmacologic DRD3-antagonism in the functional phenotype of astrocytes and microglia was determined by immunohistochemistry and flow cytometry at different time-points.

Montoya A, Elgueta D, Campos J, Chovar O, Falcón P, Matus S, Alfaro I, Bono MR, Pacheco R.

ISI

diciembre 06, 2019

DOI: J Neuroinflammation. 2019 Dec 6;16(1):258. doi: 10.1186/s12974-019-1652-8.

Investigador BNI: Soledad Matus